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discovery series pdquest 2 d analysis software  (Bio-Rad)


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    Bio-Rad discovery series pdquest 2 d analysis software
    Tissue derived from the rat SVZ (black line in Nissl stained section) is dissected free and homogenized. This area of the SVZ contains a large pool of neural stem/progenitor cells as evidenced by their staining with a nestin antibody. 2-D gels are generated from this tissue and protein expression patterns are analyzed both manually and using <t>PDQuest</t> 2D analysis software. Using trypsin as an enzymatic digest, proteins of interest are cleaved immediately downstream of the amino acids arginine and lysine, thereby generating peptide fragments of varying molecular weights. These peptide fragments are then subjected to LC-ESI-MS/MS to obtain amino acid sequence information, which is subsequently compared to theoretical mass spectra using a MASCOT database search in order to determine the identity of the protein.
    Discovery Series Pdquest 2 D Analysis Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/discovery+series+pdquest+2+d+software/PDQuest+Advanced+2-D+Analysis+Software/pmc03323769-145-19-25
    Average 96 stars, based on 3299 article reviews
    discovery series pdquest 2 d analysis software - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Age-related proteomic changes in the subventricular zone and its association with neural stem/progenitor cell proliferation"

    Article Title: Age-related proteomic changes in the subventricular zone and its association with neural stem/progenitor cell proliferation

    Journal: Journal of Neuroscience Research

    doi: 10.1002/jnr.23012

    Tissue derived from the rat SVZ (black line in Nissl stained section) is dissected free and homogenized. This area of the SVZ contains a large pool of neural stem/progenitor cells as evidenced by their staining with a nestin antibody. 2-D gels are generated from this tissue and protein expression patterns are analyzed both manually and using PDQuest 2D analysis software. Using trypsin as an enzymatic digest, proteins of interest are cleaved immediately downstream of the amino acids arginine and lysine, thereby generating peptide fragments of varying molecular weights. These peptide fragments are then subjected to LC-ESI-MS/MS to obtain amino acid sequence information, which is subsequently compared to theoretical mass spectra using a MASCOT database search in order to determine the identity of the protein.
    Figure Legend Snippet: Tissue derived from the rat SVZ (black line in Nissl stained section) is dissected free and homogenized. This area of the SVZ contains a large pool of neural stem/progenitor cells as evidenced by their staining with a nestin antibody. 2-D gels are generated from this tissue and protein expression patterns are analyzed both manually and using PDQuest 2D analysis software. Using trypsin as an enzymatic digest, proteins of interest are cleaved immediately downstream of the amino acids arginine and lysine, thereby generating peptide fragments of varying molecular weights. These peptide fragments are then subjected to LC-ESI-MS/MS to obtain amino acid sequence information, which is subsequently compared to theoretical mass spectra using a MASCOT database search in order to determine the identity of the protein.

    Techniques Used: Derivative Assay, Staining, Generated, Expressing, Software, Tandem Mass Spectroscopy, Sequencing

    Proteins resolved on 2D gels that exhibit greater than a three-fold change in expression level with aging in the SVZ were identified using LC-ESI-MS/MS. To control for loading and running conditions between gels, 50ng of a purified tropomyosin standard was added to each sample preparation. The fold change in expression of a protein with aging was determined using PDQuest 2-D gel analysis software.
    Figure Legend Snippet: Proteins resolved on 2D gels that exhibit greater than a three-fold change in expression level with aging in the SVZ were identified using LC-ESI-MS/MS. To control for loading and running conditions between gels, 50ng of a purified tropomyosin standard was added to each sample preparation. The fold change in expression of a protein with aging was determined using PDQuest 2-D gel analysis software.

    Techniques Used: Expressing, Tandem Mass Spectroscopy, Control, Purification, Sample Prep, Software

    Related Articles

    Staining:

    Article Title: Enhanced Expression of the Voltage-Dependent Anion Channel 1 (VDAC1) in Alzheimer's Disease Transgenic Mice: An Insight into the Pathogenic Effects of Amyloid-β
    Article Snippet: The first phase was set at 85 V for 12 h and the second phase was set at 300 V for 1 h. The proteins of the 2D-gels obtained were detected using a colloidal Coomassie G-250 staining (Bio-Rad, CA, USA). .. The stained gels were digitized with a GS-800 calibrated densitometer (Bio-Rad, CA, USA) and analyzed with the Discovery Series PDQuest 2-D software (Bio-Rad, CA, USA). ..

    Software:

    Article Title: Enhanced Expression of the Voltage-Dependent Anion Channel 1 (VDAC1) in Alzheimer's Disease Transgenic Mice: An Insight into the Pathogenic Effects of Amyloid-β
    Article Snippet: The first phase was set at 85 V for 12 h and the second phase was set at 300 V for 1 h. The proteins of the 2D-gels obtained were detected using a colloidal Coomassie G-250 staining (Bio-Rad, CA, USA). .. The stained gels were digitized with a GS-800 calibrated densitometer (Bio-Rad, CA, USA) and analyzed with the Discovery Series PDQuest 2-D software (Bio-Rad, CA, USA). ..



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    Tissue derived from the rat SVZ (black line in Nissl stained section) is dissected free and homogenized. This area of the SVZ contains a large pool of neural stem/progenitor cells as evidenced by their staining with a nestin antibody. 2-D gels are generated from this tissue and protein expression patterns are analyzed both manually and using <t>PDQuest</t> 2D analysis software. Using trypsin as an enzymatic digest, proteins of interest are cleaved immediately downstream of the amino acids arginine and lysine, thereby generating peptide fragments of varying molecular weights. These peptide fragments are then subjected to LC-ESI-MS/MS to obtain amino acid sequence information, which is subsequently compared to theoretical mass spectra using a MASCOT database search in order to determine the identity of the protein.
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    Image Search Results


    Tissue derived from the rat SVZ (black line in Nissl stained section) is dissected free and homogenized. This area of the SVZ contains a large pool of neural stem/progenitor cells as evidenced by their staining with a nestin antibody. 2-D gels are generated from this tissue and protein expression patterns are analyzed both manually and using PDQuest 2D analysis software. Using trypsin as an enzymatic digest, proteins of interest are cleaved immediately downstream of the amino acids arginine and lysine, thereby generating peptide fragments of varying molecular weights. These peptide fragments are then subjected to LC-ESI-MS/MS to obtain amino acid sequence information, which is subsequently compared to theoretical mass spectra using a MASCOT database search in order to determine the identity of the protein.

    Journal: Journal of Neuroscience Research

    Article Title: Age-related proteomic changes in the subventricular zone and its association with neural stem/progenitor cell proliferation

    doi: 10.1002/jnr.23012

    Figure Lengend Snippet: Tissue derived from the rat SVZ (black line in Nissl stained section) is dissected free and homogenized. This area of the SVZ contains a large pool of neural stem/progenitor cells as evidenced by their staining with a nestin antibody. 2-D gels are generated from this tissue and protein expression patterns are analyzed both manually and using PDQuest 2D analysis software. Using trypsin as an enzymatic digest, proteins of interest are cleaved immediately downstream of the amino acids arginine and lysine, thereby generating peptide fragments of varying molecular weights. These peptide fragments are then subjected to LC-ESI-MS/MS to obtain amino acid sequence information, which is subsequently compared to theoretical mass spectra using a MASCOT database search in order to determine the identity of the protein.

    Article Snippet: All gels from a given experimental condition were digitally scanned using a GS-800 densitometer (BioRad) and analyzed using the Discovery Series PDQuest 2-D Analysis software (BioRad).

    Techniques: Derivative Assay, Staining, Generated, Expressing, Software, Tandem Mass Spectroscopy, Sequencing

    Proteins resolved on 2D gels that exhibit greater than a three-fold change in expression level with aging in the SVZ were identified using LC-ESI-MS/MS. To control for loading and running conditions between gels, 50ng of a purified tropomyosin standard was added to each sample preparation. The fold change in expression of a protein with aging was determined using PDQuest 2-D gel analysis software.

    Journal: Journal of Neuroscience Research

    Article Title: Age-related proteomic changes in the subventricular zone and its association with neural stem/progenitor cell proliferation

    doi: 10.1002/jnr.23012

    Figure Lengend Snippet: Proteins resolved on 2D gels that exhibit greater than a three-fold change in expression level with aging in the SVZ were identified using LC-ESI-MS/MS. To control for loading and running conditions between gels, 50ng of a purified tropomyosin standard was added to each sample preparation. The fold change in expression of a protein with aging was determined using PDQuest 2-D gel analysis software.

    Article Snippet: All gels from a given experimental condition were digitally scanned using a GS-800 densitometer (BioRad) and analyzed using the Discovery Series PDQuest 2-D Analysis software (BioRad).

    Techniques: Expressing, Tandem Mass Spectroscopy, Control, Purification, Sample Prep, Software